The injury occurred with roughly 3:27 left in the second quarter. Barkley caught a pass from Daniel Jones and took an awkward hit from Mike Edwards.
After being looked at on the sideline, the 22-year-old Barkley needed help getting to the locker room.
The Giants initially deemed Barkley questionable to return, but was later downgraded to out. He returned to the Giants’ sideline wearing a walking boot and using crutches. X-Rays showed a sprained ankle, but no broken bones.
After the game, the Giants revealed Barkley is dealing with a high-ankle sprain. Barkley will undergo an MRI on Monday. While he did not break any bones, Barkley is expected to miss time.
Barkley finished the game with eight carries for 10 yards and four receptions for 27 yards.
More from Yahoo Sports: