Outside linebacker James Harrison, safety Troy Polamalu and running back Rashard Mendenhall have been ruled out of Sunday's game at Oakland.
Harrison has not played since undergoing knee surgery Aug. 15, while Polamalu missed last week's victory over the Jets with a calf injury. Mendenhall is continuing his recovery from January knee surgery.
All three are expected to return following the team's Week 4 bye, when the Steelers will play twice in five days.
Chris Carter will continue to replace Harrison, while Ryan Mundy will start in the seconary next to Ryan Clark.
Tight end Heath Miller (rib cage soreness) is expected to play at Oakland.