Djokovic, the 2011 Wimbledon champion, is ranked No. 2 but was given the top seeding by the All England Club ahead of top-ranked Rafael Nadal.
Nadal, a two-time Wimbledon champion who is coming off his ninth French Open title, is seeded No. 2 for the grass-court Grand Slam, which starts on Monday.
Murray last year became the first British player to win the Wimbledon men's title since 1936.
Seven-time champion Roger Federer is No. 4, while Australian Open winner Stan Wawrinka is down two spots from his world ranking at No. 5.
Tomas Berdych, David Ferrer, Milos Raonic, John Isner and Kei Nishikori round out the top 10.
Among the 32 seeded players, Jerzy Janowicz received the biggest boost, going up nine spots from his No. 24 ranking to No. 15. The big-serving Polish player reached the Wimbledon semifinals last year, losing to Murray in four sets.
Wimbledon takes a player's grass-court record into account in assigning the men's seedings.
The women's seedings, however, stick to the WTA rankings.
That means five-time champion Williams is No. 1, followed by Li Na, French Open runner-up Simona Halep, Agnieszka Radwanska and Maria Sharapova.
Sharapova, who won her first Grand Slam title at Wimbledon 10 years ago, is coming off her second French Open championship. She is considered Williams' top challenger at Wimbledon, despite the No. 5 seeding. They could end up on the same side of the draw.
Petra Kvitova, the 2011 Wimbledon winner, is No. 6, followed by Jelena Jankovic, Victoria Azarenka, Angelique Kerber and Dominika Cibulkova.
Venus Williams, a five-time Wimbledon champion who has gradually slipped in the rankings, is seeded only No. 30.