The No. 10-seeded Konta, the first British woman to reach a Key Biscayne final, was the more aggressive player from the start and finished with 33 winners, compared with eight for the 12th-seeded Wozniacki.
Konta showed her versatility on the final point, drawing Wozniacki to the net with a drop shot and then floating a lob off the back of the baseline for the clinching winner.
She improved to 19-3 this year. Her other titles came at Sydney this year and Stanford in 2016.
The 25-year-old Konta received $1.18 million and will climb to a career-high No. 7 next week.