Jones and Romero were also left out of United’s Champions League squad earlier this month after falling out of favour under Ole Gunnar Solskjaer.
But Marcos Rojo, who was also omitted from the Champions League list, will be available for selection domestically after being included in the Premier League list.
Jones made just eight appearances for United last season, enduring yet another campaign blighted by injury and fitness concerns. The 28-year-old signed a a four-year contract at Old Trafford only last year.
Romero, meanwhile, has not returned to training at United’s Carrington base this season after falling down the pecking order.
The Argentina international was Solskjaer’s second-choice goalkeeper until the return of Dean Henderson from a loan spell at Sheffield United.
Romero hoped to secure a move away from United during the transfer window, with Everton and Aston Villa interested in his services, but ultimately stayed put.
United’s treatment of Romero was publicly criticised by his wife Eliana Guercio, who accused the Old Trafford club of lacking respect.
Manchester United’s 25-man Premier League squad
Main list: Eric Bailly, Edinson Cavani, David de Gea, Bruno Fernandes, Fred, Odion Ighalo, Victor Lindelof, Anthony Martial, Juan Mata, Nemanja Matic, Marcos Rojo, Alex Telles, Donny van de Beek
Homegrown: Timothy Fosu-Mensah, Lee Grant, Dean Henderson, Daniel James, Jesse Lingard, Harry Maguire, Scott McTominay, Paul Pogba, Marcus Rashford, Luke Shaw, Axel Tuanzebe, Aaron Wan-Bissaka
Under-21: Mason Greenwood, Teden Mengi, Facundo Pellistri, Brandon Williams