The team says Chamberlain struck out two and allowed an unearned run Tuesday in rookie ball against the GCL Pirates, his first game since injuring his ankle during spring training.
Chamberlain dislocated his right ankle bouncing on a trampoline with his son on March 22. The right-hander was coming back from elbow ligament replacement surgery at the time of the injury.
The 26-year-old Chamberlain has not pitched in the majors since June 5, 2011. He was 2-0 with a 2.83 ERA in 27 appearances last year.