Michal Bilek has no fresh injury concerns ahead of the qualifier and could field the same XI that comfortably saw off the challenge of Armenia with a 3-0 away win back in March.
Matej Vydra scored two goals in that match and is set to lead the line once again. Arsenal midfielder Tomas Rosicky will look to add some creative spark from midfield, while Chelsea goalkeeper Petr Cech closes in on Karel Poborksy's appearance record (118) with his 101st cap.
Cesare Prandelli can welcome back Antonio Barzagli and Stephan El Shaarawy after the pair missed the 4-0 friendly victory over San Marino last week, and both are expected to go straight into the starting lineup.
Prandelli is expected to stick with the 4-3-3 formation from the last match with Ignazio Abate vying with Christian Maggio for the right back spot. Torino winger Alessio Cerci could be handed a rare start on the right wing with the Azzurri's top goalscorer in qualifying, Mario Balotelli, in the lone striking role.
There is no spot for Genoa defender Luca Antonelli, who has been ruled out through an ankle injury.