The Spurs boss took over at White Hart Lane in the summer was talking after his side clinched a place in the last 16 thanks to some late drama against French club Lyon in the round of 32. The Premier League side was trailing 1-0 on the night and tied at 2-2 on aggregate but heading out on away goals, until Belgium international Mousa Dembele's powerful last minute strike put it through to face the Italians.
"We just want to recreate a special night everyone remembers at the club," Villas-Boas told reporters. "Going through against Inter Milan and Lyon [would] put you in a good place to win the competition."
The tie will be what many Tottenham fans will see as their biggest of the season and will evoke special memories of the last time the teams met, during the group stages of the Champions League back in 2010.
During those two games, Spurs took advantage of some sloppy and uncharacteristic defense by the Italians. They lost 4-3 in the San Siro despite a hat trick from Welsh star Gareth Bale, before the winger put in another great performance at White Hart Lane as they ran out 3-1 winners.
Bale had a far more muted performance in France and Villas-Boas admitted that his team did not play well in the first half in Lyon to defend their 2-1 advantage from the first leg, as a Maxime Gonalons goal threatened to know the north Londoners out of the competition before Dembele's goal.
"In the second half we were the better team," Villas-Boas added. "We created some chances and we were rewarded with the second goal in the end. Individual players make a difference and Mousa certainly made the difference.
"If you look at the number of chances Lyon had over the two legs it would not have been fair if they had gone through."