The Wales international sat out last weekend's Liga meeting with Athletic Bilbao because of a calf problem but it had been hoped that he would be available to face the Rojiblancos.
However, the game at the Santiago Bernabeu has come too soon for Bale, who has been plagued by injuries since arriving at Madrid from Tottenham last summer.
The attacker's absence means that the in-form Jese Rodriguez is likely to start up front alongside Karim Benzema and Cristiano Ronaldo, who is free to play as his three-game ban for being sent off at San Mames will only end up covering Liga matches.
- Sports & Recreation
- Athletic Bilbao