The Slovakian has not traveled with his side as they flew out to Prague on Wednesday ahead of the last-32 clash at Struncovy Sady after suffering with the flu virus for the past few days.
Viktoria holds a 3-0 lead from the first leg after a shock victory at the San Paolo last week, thanks to goals from Vladimir Darida, Frantisek Rajtoral and Stanislav Tecl.
Hamsik was the victim of armed robbery in Naples earlier this week when the window of his vehicle was smashed by thieves, who made off with the playmaker’s Rolex watch.
The 25-year-old was unharmed in the incident.
Follow GOAL.COM on Twitter
- Sports & Recreation
- Morgan De Sanctis
- Hugo Campagnaro
- Paolo Cannavaro