Samantha Prahalis had 19 points and reserve forward Darryce Moore finished with 10 for the Buckeyes (11-0), who have won 22 of 23 dating to last season.
Hillary Sigmon had 14 points and Gabby Tyler scored 13 for the 49ers (5-5), who have lost five straight. It was Charlotte’s first game since Dec. 7.
The Buckeyes showed little signs of fatigue from a red-eye flight Saturday night after their 77-75 win at California. They jumped out to a 44-14 lead.